The large amount of existing nanomaterials demands for rapid and reliable ways to test their potential toxicological effect on human health, preferably by means of relevant in vitro tests in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows to derive much more information from cell-based assays than with the typical readouts (i.e. one measurement per well) with optical plate-readers. We present here a dataset including up to 14 different read outs including viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential and steatosis of the human hepatoma HepaRG cell line treated with a large set of nanomaterials, coatings and supernatants at different concentrations. The database, given its size, can be utilized for the development of in silico hazard assessment and prediction tools or can be combined with toxicity effect on other in vitro test systems.
Douglas Gilliland; Elisabeth Joossens
; Eugenia Valsami-Jones; Isaac Ojea-jiménez; Isabella Römer; Iseult Lynch; Jean-Michel Gineste; Kirsten Britta Gerloff; Maurice Whelan; Peter Macko; Salvador Fortaner Torrent; Sophie Marie Briffa; Taina Palosaari
Joossens, Elisabeth; Macko, Peter; Palosaari, Taina; Gerloff, Kirsten Britta; Ojea-jiménez, Isaac; Gilliland, Douglas; Fortaner Torrent, Salvador; Gineste, Jean-Michel; Römer, Isabella; Briffa, Sophie Marie; Valsami-Jones, Eugenia; Lynch, Iseult; Whelan, Maurice (2026): HTS DB of Nanomaterials on HepaRGs. European Commission, Joint Research Centre [Dataset] doi: 10.2905/JRC.HZB8140; 10.2905/jrc-10088-10009 PID: http://data.europa.eu/89h/jrc-10088-10009
The Excel file contains the original database.
The Zip file contains the following elements: (1) investigation.xlsx, a file with metadata of the experiments, assay specific information, the experimental plate layouts and NM characterisation; (2) methods containing the data acquisition codes and the dispertion protocols; (3) RawData.zip and (4) TransformedData.zip, two zip archives with the original and processed data.
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The large amount of existing nanomaterials demands rapid and reliable methods for testing their potential toxicological effect on human health, preferably by means of relevant in vitro techniques in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows deriving much more information from cell-based assays than the typical readouts (i.e. one measurement per well) with optical plate-readers. We present here a dataset including data based on a maximum of 14 different read outs (including viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential and steatosis) of the human hepatoma HepaRG cell line treated with a large set of nanomaterials, coatings and supernatants at different concentrations. The database, given its size, can be utilized in the development of in silico hazard assessment and prediction tools or can be combined with toxicity results from other in vitro test systems.
| From date | To date |
|---|---|
| 2014-02-13 | 2016-06-27 |
