DATASET

HTS DB of Nanomaterials on HepaRGs

Collection: EURL-ECVAM : European Union Reference Laboratory for Alternatives to Animal Testing 

Description

The large amount of existing nanomaterials demands for rapid and reliable ways to test their potential toxicological effect on human health, preferably by means of relevant in vitro tests in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows to derive much more information from cell-based assays than with the typical readouts (i.e. one measurement per well) with optical plate-readers. We present here a dataset including up to 14 different read outs including viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential and steatosis of the human hepatoma HepaRG cell line treated with a large set of nanomaterials, coatings and supernatants at different concentrations. The database, given its size, can be utilized for the development of in silico hazard assessment and prediction tools or can be combined with toxicity effect on other in vitro test systems.

Contact

Email
jrc-f3-enquiries (at) ec.europa.eu

Contributors

  • Elisabeth Joossens
    0000-0001-8091-4192
  • Peter Macko
  • Taina Palosaari
  • Kirsten Britta Gerloff
  • Isaac Ojea-jiménez
  • Douglas Gilliland
  • Salvador Fortaner Torrent
  • Jean-Michel Gineste
  • Isabella Römer
  • Sophie Marie Briffa
  • Eugenia Valsami-Jones
    0000-0002-8850-7556
  • Iseult Lynch
    0000-0003-4250-4584
  • Maurice Whelan

How to cite

Joossens, Elisabeth; Macko, Peter; Palosaari, Taina; Gerloff, Kirsten Britta; Ojea-jiménez, Isaac; Gilliland, Douglas; Fortaner Torrent, Salvador; Gineste, Jean-Michel; Römer, Isabella; Briffa, Sophie Marie; Valsami-Jones, Eugenia; Lynch, Iseult; Whelan, Maurice (2018): HTS DB of Nanomaterials on HepaRGs. European Commission, Joint Research Centre (JRC) [Dataset] doi: 10.2905/jrc-10088-10009 PID: http://data.europa.eu/89h/jrc-10088-10009

Keywords

HepaRG Nanomaterials Toxicity

Data access

NanoMILE HTS HepaRG
Download 
  • The Excel file contains the original database.

Background Information
Download 
  • The Zip file contains the following elements: (1) investigation.xlsx, a file with metadata of the experiments, assay specific information, the experimental plate layouts and NM characterisation; (2) methods containing the data acquisition codes and the dispertion protocols; (3) RawData.zip and (4) TransformedData.zip, two zip archives with the original and processed data.

Publications

Publication
A high throughput imaging database of toxicological effects of nanomaterials tested on HepaRG cells
Joossens, E., Macko, P., Palosaari, T., Gerloff, K., Ojea Jimenez, I., Gilliland, D., Novak, J., Fortaner Torrent, S., Gineste, J., Romer, I., Briffa, S., Valsami-Jones, E., Lynch, I. and Whelan, M., A high throughput imaging database of toxicological effects of nanomaterials tested on HepaRG cells, SCIENTIFIC DATA, ISSN 2052-4463 (online), 6 (46), 2019, p. 1-10, JRC114500.
  • NATURE PUBLISHING GROUP, LONDON, ENGLAND
Publication page 
  • Abstract

    The large amount of existing nanomaterials demands rapid and reliable methods for testing their potential toxicological effect on human health, preferably by means of relevant in vitro techniques in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows deriving much more information from cell-based assays than the typical readouts (i.e. one measurement per well) with optical plate-readers. We present here a dataset including data based on a maximum of 14 different read outs (including viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential and steatosis) of the human hepatoma HepaRG cell line treated with a large set of nanomaterials, coatings and supernatants at different concentrations. The database, given its size, can be utilized in the development of in silico hazard assessment and prediction tools or can be combined with toxicity results from other in vitro test systems.

Geographic areas

World

Temporal coverage

From date To date
2014-02-13 2016-06-27

Additional information

Published by
European Commission, Joint Research Centre
Created date
2018-12-14
Modified date
2019-05-15
Issued date
2018-03-16
Landing page
https://ec.europa.eu/jrc/en/research-topic/alternatives-animal-testing-and-safety-assessment-chemicals 
Language(s)
English
Data theme(s)
Science and technology
Update frequency
irregular
Identifier
http://data.europa.eu/89h/jrc-10088-10009
Popularity