Raw sequence data of genetically modified strains of Bacillus subtilis used for production of vitamin B2 (riboflavin) in feed additive. The collection contains three genomes sequenced at Joint Research Centre (JRC), Hessian State laboratory (LHL) and Bavarian Health and Food Safety Authority (LGL). This collection has been used for the molecular characterization of this unauthorized genetically modified Bacillus subtilis production strain. In case of reuse please cite https://doi.org/10.1016/j.foodchem.2017.03.042
- Lutz Grohmann
How to cite
Meinel, Dominik M.; Paracchini, Valentina; Angers-Loustau , Alexandre; Petrillo, Mauro; Reiting, Ralf; Wahler, Daniela; Stolz, Andrea; Schönig, Birgit; Matthies, Anastasia; Bendiek, Joachim; Pecoraro, Sven; Patak, Alex; Kreysa, Joachim; Grohmann, Lutz (2014): GM Bacillus subtilis. European Commission, Joint Research Centre (JRC) [Dataset] PID: http://data.europa.eu/89h/2abb5c2b-3ab6-4ce4-b103-cb1c5fc7349e
Next Generation Sequencing, genetically modified organisms (GMO), Genetically modified microorganisms (GMM), Whole Genome Sequencing, Bacteria, Riboflavin, Vitamin B2, Polymerase chain reaction (PCR), Feed additives, Omics, Genomics
GM Bacillus Subtilis sequenced at
Bavarian Health and Food Safety Authority (LGL) in Germany using a Illumina HiSeq 1500 device.
GM Bacillus Subtilis sequenced at Joint Research Centre (JRC) of the European Commission (Italy) with GS Junior System, 454 Life Sciences, Roche Applied Sciences.
GM Bacillus Subtilis sequenced at Hessian State laboratory (LHL) using a MiSeq apparatus (Illumina Inc.)
- ELSEVIER SCI LTD, OXFORD, ENGLAND
Many food and feed additives result from fermentation of genetically modified (GM) microorganisms. For vitamin B2 (riboflavin), GM Bacillus subtilis production strains have been developed and are often used. The presence of neither the GM strain nor its recombinant DNA is allowed for fermentation products placed on the EU market as food or feed additive. A vitamin B2 product (80% feed grade) imported from China was analysed. Viable B. subtilis cells were identified and DNAs of two bacterial isolates (LHL and LGL) were subjected to three whole genome sequencing (WGS) runs with different devices (MiSeq, 454 or HiSeq system). WGS data revealed the integration of a chloramphenicol resistance gene, the deletion of the endogenous riboflavin (rib) operon and presence of four putative plasmids harbouring rib operons. Event- and construct-specific real-time PCR methods for detection of the GM strain and its putative plasmids in food and feed products have been developed.
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- European Commission, Joint Research Centre
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- Data theme(s)
- Agriculture, fisheries, forestry and food
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