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GM Bacillus subtilis

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Raw sequence data of genetically modified strains of Bacillus subtilis used for production of vitamin B2 (riboflavin) in feed additive. The collection contains three genomes sequenced at Joint Research Centre (JRC), Hessian State laboratory (LHL) and Bavarian Health and Food Safety Authority (LGL).

This collection has been used for the molecular characterization of this unauthorized genetically modified Bacillus subtilis production strain.

In case of reuse please cite https://doi.org/10.1016/j.foodchem.2017.03.042

Contributors

Alex Patak; Alexandre Angers-Loustau; Anastasia Matthies; Andrea Stolz; Birgit Schönig; Daniela Wahler; Dominik M. Meinel; Joachim Bendiek; Joachim Kreysa; Lutz Grohmann; Mauro Petrillo; Ralf Reiting; Sven Pecoraro; Valentina Paracchini

How to cite

Paracchini, Valentina; Petrillo, Mauro; Reiting, Ralf; Angers-Loustau, Alexandre; Wahler, Daniela; Stolz, Andrea; Schönig, Birgit; Matthies, Anastasia; Bendiek, Joachim; Meinel, Dominik; Pecoraro, Sven; Busch, Ulrich; Patak, Alex; Kreysa, Joachim; Grohmann, Lutz (2026): GM Bacillus subtilis. European Commission, Joint Research Centre [Dataset] doi: 10.2905/JRC.91BGCZ0 PID: http://data.europa.eu/89h/2abb5c2b-3ab6-4ce4-b103-cb1c5fc7349e

Keywords

BacteriaFeed additivesGenetically modified microorganisms (GMM)genetically modified organisms (GMO)GenomicsNext Generation SequencingOmicsPolymerase chain reaction (PCR)RiboflavinVitamin B2Whole Genome Sequencing

Data access

GM Bacillus Subtilis - JRC
TAR GZ

TAR – tape archive – is a computer software utility for collecting many files into one archive file, often referred to as a tarball, for distribution or backup purposes. GZ – gzip – is a file format and a software application used for file compression and decompression.
Downloadable file

A downloadable file for the dataset.

Use conditions
European Commission reuse notice

According to the European Commission reuse notice, reuse is authorised, provided the source is acknowledged. The reuse policy of the European Commission is implemented by the Decision of 12 December 2011. The general principle of reuse can be subject to conditions which may be specified in individual copyright notices. Therefore users are advised to refer to the copyright notices of the individual websites maintained under Europa and of the individual documents. Reuse is not applicable to documents subject to intellectual property rights of third parties.

Access conditions
No limitations

Anybody can directly and anonymously access the data, without being required to register or authenticate.

  • GM Bacillus Subtilis sequenced at Joint Research Centre (JRC) of the European Commission (Italy) with GS Junior System, 454 Life Sciences, Roche Applied Sciences.

GM Bacillus Subtilis - LHL
TAR GZ

TAR – tape archive – is a computer software utility for collecting many files into one archive file, often referred to as a tarball, for distribution or backup purposes. GZ – gzip – is a file format and a software application used for file compression and decompression.
Downloadable file

A downloadable file for the dataset.

Use conditions
European Commission reuse notice

According to the European Commission reuse notice, reuse is authorised, provided the source is acknowledged. The reuse policy of the European Commission is implemented by the Decision of 12 December 2011. The general principle of reuse can be subject to conditions which may be specified in individual copyright notices. Therefore users are advised to refer to the copyright notices of the individual websites maintained under Europa and of the individual documents. Reuse is not applicable to documents subject to intellectual property rights of third parties.

Access conditions
No limitations

Anybody can directly and anonymously access the data, without being required to register or authenticate.

  • GM Bacillus Subtilis sequenced at Hessian State laboratory (LHL) using a MiSeq apparatus (Illumina Inc.)

GM Bacillus Subtilis - LGL
TAR GZ

TAR – tape archive – is a computer software utility for collecting many files into one archive file, often referred to as a tarball, for distribution or backup purposes. GZ – gzip – is a file format and a software application used for file compression and decompression.
Downloadable file

A downloadable file for the dataset.

Use conditions
European Commission reuse notice

According to the European Commission reuse notice, reuse is authorised, provided the source is acknowledged. The reuse policy of the European Commission is implemented by the Decision of 12 December 2011. The general principle of reuse can be subject to conditions which may be specified in individual copyright notices. Therefore users are advised to refer to the copyright notices of the individual websites maintained under Europa and of the individual documents. Reuse is not applicable to documents subject to intellectual property rights of third parties.

Access conditions
No limitations

Anybody can directly and anonymously access the data, without being required to register or authenticate.

  • GM Bacillus Subtilis sequenced at Bavarian Health and Food Safety Authority (LGL) in Germany using a Illumina HiSeq 1500 device.

Publications

Publication
Molecular characterization of an unauthorized genetically modified Bacillus subtilis production strain identified in a vitamin B2 feed additive
Paracchini V; Petrillo M; Reiting R; Angers A; Wahler D; Stolz A; Schönig B; Matthies A; Joachim B; Dominik M. M; Pecoraro S; Busch U; Patak Dennstedt A; Kreysa J; Grohmann L. Molecular characterization of an unauthorized genetically modified Bacillus subtilis production strain identified in a vitamin B2 feed additive. FOOD CHEMISTRY 230; 2017. p. 681-689. JRC103393
ELSEVIER SCI LTD, OXFORD, ENGLAND
DOI badgeISSN badge

  • Many food and feed additives result from fermentation of genetically modified (GM) microorganisms. For vitamin B2 (riboflavin), GM Bacillus subtilis production strains have been developed and are often used. The presence of neither the GM strain nor its recombinant DNA is allowed for fermentation products placed on the EU market as food or feed additive. A vitamin B2 product (80% feed grade) imported from China was analysed. Viable B. subtilis cells were identified and DNAs of two bacterial isolates (LHL and LGL) were subjected to three whole genome sequencing (WGS) runs with different devices (MiSeq, 454 or HiSeq system). WGS data revealed the integration of a chloramphenicol resistance gene, the deletion of the endogenous riboflavin (rib) operon and presence of four putative plasmids harbouring rib operons. Event- and construct-specific real-time PCR methods for detection of the GM strain and its putative plasmids in food and feed products have been developed.

Additional information

Published by
European Commission, Joint Research Centre
Contact email
alex.patak (at) ec.europa.eu
Update frequency
unknown

The event occurs with unknown regularity.

Language(s)
English

English is a member of the West Germanic group of the Germanic languages. It is an official language of almost 60 sovereign states and is now a global lingua franca.It is the third-most-common native language in the world and it is widely learned as a second language.

Data theme(s)
Agriculture, fisheries, forestry and food

dataset theme covering the domains of agriculture which involves the cultivation of plants and livestock; fisheries which focuses on the harvesting of fish from wild or farmed sources; forestry which relates to the management and conservation of forests and woodlands; and food which includes substances that provide nutritional support to organisms

Issued date
2014-01-01
Created date
21 Jun 2019 14:17
Modified date
29 Jan 2021 10:14
Dataset identifier
http://data.europa.eu/89h/2abb5c2b-3ab6-4ce4-b103-cb1c5fc7349e
Other identifiers
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