Description
The toxicological response of a metabolic competent cell line (HepaRG) is investigated combining a high throughput workflows with automated high content imaging approach.
Nine different read outs related to cell viability and mitochondrial damage are measured simultaneously after exposing the cells for 24 hours to increasing concentrations of five chemicals: with aflatoxin B1, benzo[a]pyrene, cyclosporin A, rotenone and thrichostatin A.
Contact
Contributors
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- Donatella Carpi
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- Taina Palosaari
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- Peter Macko
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- Roman Liska
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- Julia Malinowska
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- Mounir Bouhifd
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- Maurice Whelan
How to cite
Carpi, Donatella; Palosaari, Taina; Macko, Peter; Liska, Roman; Malinowska, Julia; Bouhifd, Mounir; Whelan, Maurice (2018): High Content Imaging analysis of HepaRG cell line treated with aflatoxin B1, benzo[a]pyrene, cyclosporin A, rotenone and thrichostatin A over a range of chemical concentrations. European Commission, Joint Research Centre (JRC) [Dataset] doi: 10.2905/8b23d04a-63e2-40c0-9569-b20813253b0b PID: http://data.europa.eu/89h/8b23d04a-63e2-40c0-9569-b20813253b0b
Keywords
in vitro experiments toxicology
Data access
EURL ECVAM HCI imaging facility objectives
High Content Imaging of the HepaRG cell line treated with aflatoxin B1, benzo[a]pyrene, cyclosporin A, rotenone and trichostatin A over a range of chemical concentrations
Additional information
- Published by
- European Commission, Joint Research Centre
- Created date
- 2023-06-26
- Modified date
- 2023-12-12
- Issued date
- 2018-03-01
- Landing page
- https://joint-research-centre.ec.europa.eu/eu-reference-laboratory-alternatives-animal-testing-eurl-ecvam_en
- Data theme(s)
- Science and technology
- Update frequency
- irregular
- Identifier
- http://data.europa.eu/89h/8b23d04a-63e2-40c0-9569-b20813253b0b
- Popularity
-